Unity through Knowledge Fund 3B Project Report
Project title: Exercise-induced improvement of chronic heart failure: the role of KATP channels and mitochondria
Project leaders: Marko Ljubković, MD, PhD and Jasna Marinović, MD, PhD
Duration of the project: 24 months
Call: 3B – Reintegration Grant
Home institution: University of Split School of Medicine
Project budget: 709.780 HRK
Other organizations involved:
Department of Anesthesiology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI,USA, http://www.mcw.edu/anesthesiology.htm
Department of Circulation and Medical Imaging, Norwegian University of Science and Technology, Olav Kyrres gt. 9, 7489 Trondheim, Norway, http://www.ntnu.no/isb
Project summary: The project entitled “Exercise-induced improvement of chronic heart failure: the role of KATP channels and mitochondria” was awarded to Drs. Marko Ljubkovic and Jasna Marinovic from the Unity through Knowledge Fund of the Croatian Ministry of Science. The main goal of the Project is to investigate the specific contribution of mitochondria and ion channels in the beneficial mechanisms whereby different modes of exercise improve the function of the previously failing heart. The project commenced in 2009 and is conducted in collaboration with Dr. Ulrik Wisloff from the Department of Circulation and Medical Imaging from the Norwegian University of Science and Technology (Trondheim, Norway) and Dr. Martin Bienengraeber from the Department of Anesthesiology from the Medical College of Wisconsin (Milwaukee, WI, USA).
- The procedure of coronary artery ligation surgery for the purpose of inducing chronic heart failure in experimental animals is fully established with over 150 animals operated in the period from June 2010 – May 2011. The efficacy in inducing heart failure is ≈50 %.
- Exercise training (treadmill running) is performed daily in experimental animals with heart failure.
- Echocardiographic assessment of heart function in experimental animals has been fully established.
- The procedure for isolation of rat cardiac myocytes was fully established.
- Measurements of mitochondrial respiration, mitochondrial ATP production and cardiomyocytes survival are fully established.
- Equipment acquired through the current project: luminometer, system for assessment of cardiomyocytes viability, system for western blot analysis, system for isoflurane anesthesia of small animals, surgical instruments, reagents.
- Two projects submitted to international funding agencies.
- One abstract accepted to European Society of Cardiology Annual Meeting (will be presented in August 2011) and one manuscript in preparation pertaining to micohondrial function in animals with heart failure. We reported that rate of mitochondrial respiration and phosphorylation coupling is impaired in mitochondria isolated from the failing myocardium of ischemic etiology. Exercise training lasting for 8 weeks significantly improved the mitochondrial function, restoring the respiratory rate and increasing the level of phosphorylation efficiency.
- A visit from Dr. Martin Bienengraeber from Medical College of Wisconsin took place from May 4th-8th, 2011.
- A visit of Dr. Ljubkovic and Dr. Marinovic to Dr. Ulrik Wisloff at Norwegian University of Science and Technology took place from May 19th to 28th, 2011.
Involved personnel from MEFST:
- Dr. Marko Ljubkovic: principal investigator
- Dr. Jasna Marinovic: principal investigator
- Dr. Jasenka Kraljevic: graduate student
- Dr. Danijel Pravdic: visiting scientist
- Dr. Petra Zubin: graduate student
- Ivana Banic: laboratory technician
- Dr. Ulrik Wisloff (NTNU)
- Dr. Martin Bienengraeber (MCW)